Fozol Korim Ovi contributed in data analysis, statistics, and writing of the manuscript. Aoulad Hosen contributed as first author and was responsible for conception, experiment design, data collection and writing of the manuscript. The authors declare that there is no conflict of interest. All authors listed in this paper, was fully involved in the research and they contributed their full efforts, and revised the manuscript before final submission. doi: 10.3934/microbiol.2021028ĭepartment of Microbiology, Gono Bishwabidalay, Savar, Bangladesh, supported this research project. in environmental water samples collected from flood prone areas of Bangladesh and their antibiotic resistance profile. Abdul Khalek, Mahmudul Hasan, Farhana Easmin, Nazmi Ara Rumi, Mohammad Shariful Islam. Aoulad Hosen, Fozol Korim Ovi, Harunur Rashid, MD. Furthermore, we suggest selective use of sensitive antimicrobials listed here for therapeutics of cholera outbreak.Ĭitation: Md. Based on these data we recommend using tube-well water instead of river and pond water for drinking purposes. Isolates showed marginal sensitivity towards Tetracycline (33.33%), and Cephalexin (16.67%) and 100% resistance against antibiotics like Vancomycin, Penicillin, Erythromycin, and Nalidixic Acid. All the 12 isolates were sensitive to Gentamicin and ciprofloxacin (100%), while Chloramphenicol (91.67%), Sulfamethoxazole (91.67%), Azithromycin (66.67%) showed high sensitivity. than river and pond water (P 0.05) significantly in 5 different location the sample was collected from. Tube-well water has significantly lower concentration (log CFU/mL) of V. The results showed, out of 45 samples 12 contained V. cholera isolates to determine their antibiotic sensitivity profile. Following isolation antibiotic sensitivity test was performed on each V. cholera and was confirmed by analyzing their biochemical characteristics like Catalase, Oxidase, MR, VP, Indole, Sugar fermentation. Yellow colonies on TCBS agar were screed as V. cholera isolation water samples were first enriched in nutrient broth at 37 ☌ for 16 hours followed by cultivation in selective media TCBS agar at 37 ☌ for 24 hours. Samples are then serially diluted in alkaline peptone water and streak on Thiosulfate Citrate Bile Salt Sucrose-TCBS agar for quantification of V. A total of 45 water samples were randomly collected for the isolation and identification of Vibrio spp. cholera in 3 different water sources River, pond and tube-well, in 5 different locations of Gazipur, Bangladesh, and to analyze their antibiogram study. ![]() Therefore, the objective of this research was to isolate V. Serotype O1 of Vibrio cholera is considered as the principal causative agent which transmits through contaminated drinking water resulting that epidemic. 20% of these isolates were resistant to kanamycin and 46.67% were resistant to oxacillin.Īntibiotic susceptibility Bacterial culture Biochemical character Disc agar diffusion Minimum inhibitory concentration Oxacillin Pathogen Staphylococcus aureus.Last cholera epidemic has been recorded in Bangladesh between 1992–1993, while few sporadic localized outbreaks have been reported as recent as 2005. These findings show that all these isolates have gelatin, urea, galactose hydrolysis and lactose fermenting activity. ![]() Antibiotic susceptibility testing suggested that 20% of isolates were resistant to kanamycin and 46.67% were resistant to oxacillin. ![]() aureus isolates showed positive results in gelatin, urea and galactose hydrolysis test, 50% isolates were positive in starch hydrolysis test, 35% in protein hydrolysis test, 100% isolates in lactose fermenting test, but no isolate was positive in sucrose fermenting test. Antibiotic susceptibility were carried out by minimum inhibitory concentration test, minium bactericidal concentration test, disc agar diffusion test and brain heart infusion oxacillin screening agar.įrom this study, it was observed that 100% S. Characters of these strains were determined by traditional biochemical tests such as hydrolysis test of gelatin, urea, galactose, starch and protein, and fermentation of lactose and sucrose. Bacterial culture was done in Mueller-Hinton broth at 37 ☌. Thirty post operative pathogenic isolated S. auerus) strains against some conventional and traditional antibiotics. To observe the biochemical characters and antibiotic susceptibility of isolated Staphylococcus aureus (S.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |